Combined reflectance confocal microscopy-optical coherence tomography for delineation of basal cell carcinoma margins: an ex vivo study.
Iftimia N, Peterson G, Chang EW, Maguluri G, Fox W, Rajadhyaksha M, J Biomed Opt. 2016 Jan 1;21(1):16006. doi: 10.1117/1.JBO.21.1.016006.
We present a combined reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) approach, integrated within a single optical layout, for diagnosis of basal cell carcinomas (BCCs) and delineation of margins. While RCM imaging detects BCC presence (diagnoses) and its lateral spreading (margins) with measured resolution of ><nobr>∼1 μm</nobr>, OCT imaging delineates BCC depth spreading (margins) with resolution of ><nobr>∼7 μm</nobr>. When delineating margins in 20 specimens of superficial and nodular BCCs, depth could be reliably determined down to ><nobr>∼600 μm</nobr>, and agreement with histology was within about ><nobr>±50 μm</nobr>.
© 2016 Society of Photo-Optical Instrumentation Engineers