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Ex Vivo Confocal Microscopy Using Fusion Mode and Digital Staining: Changing Paradigms in Histological Diagnosis.

Pérez-Anker J, Malvehy J, Moreno-Ramírez D. Actas Dermosifiliogr. 2020 Jan 17. pii: S0001-7310(19)30387-4. doi: 10.1016/j.ad.2019.05.005.

ABSTRACT

The ex vivo confocal microscope is an imaging system designed to analyze freshly excised tissue using two diode lasers with different wavelengths. The technique can dramatically reduce margin analysis times and offers a sensitivity of 88% and a specificity of 89% relative to histopathology. A new technology has recently been developed that produces images more quickly and with a higher resolution than before. By means of a fusion mode that combines simultaneously scanned fluorescence and reflectance images, it produces digitally stained images that simulate the effect of hematoxylin-eosin staining. Application of this new technology has opened the door to real-time tissue diagnostics.

Copyright © 2019 AEDV. Publicado por Elsevier España, S.L.U. All rights reserved.

KEYWORDS:Claves para escaneo; Digital hematoxylin and eosin; Digital stain; Fusion ex vivo confocal microscopy; Hematoxilina y eosina digital; Microscopia confocal ex vivo de fusión; Scanning aids; Tinción digital

PMID: 31959303 DOI: 10.1016/j.ad.2019.05.005

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